Electrochemiluminescence

Electrochemiluminescence or electrogenerated chemiluminescence (ECL) is a type of luminescence produced during electrochemical reactions in solutions. The ECL platform utilized at Cambridge Biomedical allows the detection of biomarkers in single and multiplex formats in microplate wells.

Typical applications of Electrochemiluminescence

Cytokines and chemokines
Cell signaling Pathways
Angiogensis and hypoxia markers
Growth and Vascular Factors
Metabolic Markers
Immunogenicity
Cell-Based Assays
Immunogenicity
Vaccine / Bioproduction

Advantages of ECL over conventional ELISA

High Sensitivity and Low Background because the stimulation mechanism (electrical) is decoupled from the detection signal (light)
Emission read at 620 nm, eliminates issues revolving around color quenching
Improved precision from non-separation-based ("no-wash") immunoassays due to the proximity-based nature of signal generation, where only labels near the surfaces of the electrodes are stimulated
A large assay dynamic range of five logs
The carbon electrode plate surface has a 10X greater binding capacity than conventional ELISA microplates
Multiple analytes can be run in a single well reducing required sample volume

Cambridge Biomedical is CLIA certified, CAP accredited, and provides services that support best laboratory practices and GLP compliance. We help our clients navigate through quality and regulatory requirements from preclinical to IV studies.

Service Area :

CRO SERVICES
"Very good technically. People clearly knew what they were doing - knew how to handle assays, data management."

Pharmaceutical Clinical Trial Manager